To use a plasmid to create a vaccine, what has to be true of the restriction enzyme you use?

Answers

Answer 1

Answer:  The restriction enzymes have the property of cleaving DNA molecules at a specific sequences. This restriction enzymes are used to cut DNA sequences at specific points of nucleotides. Some foreign genes are inserted into plasmids to make recombinant DNA.

Explanation: When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. Overview of the restriction cloning process

hope this helps you

Answer 2

Answer: Inactive invading virusis

Explanation:


Related Questions

Suppose you are interested in the effects on boys of different dosages of a new drug to treat ADHD. You set up an experiment to consider the factor of dosage with two levels (300 mg vs. 500 mg). Which would be the best choice for the different treatment groups of the experiment within each block?

Answers

Answer:

Four groups: no drug, placebo drug, 300 mg of new drug, 500 mg of new drug.

The different treatment groups which can be included in the experimental study within each block are at least one control group, one placebo and one treatment group.

What are the treatment groups in a study?

Treatment groups are the sets of participants in a research study who are exposed to some kind of intentional change in the independent variable of interest in the study. They are an integral part of the experimental design which helps to measure effects as well as in the establishment of causality.

In a experiment, there must be at least one control group, one placebo group, and one experimental group in which controls are the participants who are not exposed to any change, treated or experimental group are the participants who are exposed to the intentional change and placebo is the group which who are exposed to drug which is similar to the drug but inactive.

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Can someone tell me the steps for making pomade ​

Answers

Answer:

1.Boil Water. Fill your pot to about 30-50% capacity and bring to a boil.

2.Measure Ingredients. ...

3.Melt Ingredients. ...

4.Heat until your waxes and oils are fully incorporated. ...

5.Remove from heat. ...

6.Add Essential Oils. ...

7.Pour the mixture into your pomade container.

Explanation:

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Answer:

https://youtu.be/DSxfxv6Y6cw

i can not tell you steps but i have give you a link that will help you don't worry it's only for making pomade

How to get into medical school/ field

Answers

Answer:

just go for it bro

Explanation:

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A 53-year-old woman collapses while gardening. She is unresponsive, is not breathing, and does not have a pulse. A neighbor, who is an emergency medical technician, rushes to her with an AED. After the AED pads are attached to the person, the AED detects ventricular fibrillation. What is the next step when using an AED

Answers

Answer:

Ventricular fibrillation presupposes one to defibrillate by first chjarging the AED to 200 joules and asking eeverybody to clear or stay away from the victim and shock the victim.

Explanation:

John is 4 years old and in today for a well child visit. He is due for his 4-year-old immunizations. He was recently diagnosed with ALL and is being treated with chemotherapy and radiation. Which statement is true about immunizations during treatments during childhood cancer

Answers

Answer:

The attenuated virus vaccines are contraindicated incChildren who are receiving treatment for cancer.

Question 7 of 10
Kyra had two jobs last year and received two W-2 forms. On the first W-2
form, the figure in box 1 was $15,667.88. On the second W-2 form, the figure
in box 1 was $9766.24. What was Kyra's gross income from the two jobs last
year?
A. $25,434 12
B. $12,717.06
C. $5901.64
D. $6358.53
SUBMIT

Answers

Answer: $25,434.12

Explanation:

Gross income refers to the sum of all the salaries, wages, interest payments, rents, profits, and every other forms of earnings, before tax is removed.

Since the first Income earned is $15,667.88 while the second income is $9766.24, then Kyra's gross income from the two jobs will be:

= $15667.88 + $9766.24

= $25434.12

A user informs you that he or she never deletes any files he or she creates. How might this affect the operating system? It will cause the operating system to crash. It will cause the operating system to upgrade. It will cause the operating system to run faster. It will cause the operating system to run slower.

Answers

Answer:

It will cause the operating system to run slower.

It is D

Answer: I dont really get it but Its D?

Explanation:

The nursing assistant cares for Mrs. Burrell, who lives in a long-term care facility. The nursing assistant allows Mrs. Burrell to decide what she is going to wear each day. The nursing assistant then assists Mrs. Burrell with her dressing and grooming routine. Which need is the nursing assistant helping Mrs. Burrell to meet

Answers

Answer: Self-esteem needs

Explanation:

The nurse is helping Mrs. Burrell to meet her self esteem needs. Self-esteem refers to how we value and perceive ourselves. Self esteem is based on our beliefs and opinions that we've about ourselves.

According to Maslow's hierarchical organization of motives, esteem needs refer to the desire of the people to have a positive evaluation of themselves. Esteem needs encompass strength, self belief, confidence, strength, and respect from others.

sing new detection techniques, researchers have found trace amounts of various medicinal substances in lakes and rivers. Taken in large quantities, these substances could have serious health effects, but they are present in quantities far too low to cause any physiological response in people who drink the water or bathe in it. Nevertheless, medical experts contend that eliminating these trace amounts from the water will have public health benefits, since __________.

Answers

Disease-causing bacteria exposed to low concentrations of certain medicinal substances can become resistant to them. this clearly mention how eliminating these substance will have health benefits.

The observation is sometimes erratic with intravenous administration true or fa

Answers

Answer:

False.

Explanation:

Intravenous administration corresponds to the application of medication directly into the patient's vein. As with any other type of drug administration, observations about the way the drug works, the dosage size, and the drug's effects on the patient must be accurate. In the case of intravenous administration, these observations are even more accurate, since the drug acts very quickly and shows results in short periods of time.

A client is admitted to the emergency department with a diagnosis of myocardial infarction (MI). The primary health care provider (PHCP) prescribes the administration of alteplase. The registered nurse (RN) preceptor is orienting a new RN in the use of this medication. Which statement by the new RN indicates that teaching has been effective

Answers

Answer:

Alteplase busts blood clots, provides perfusion , prevents further infarction

Explanation:

A clot-busting drug called alteplase used to bust the blood clot, provides perfusion , prevents further infarction. Thus its is the best statement which shows the effectiveness of RN's teaching. Thus option A is correct.

What is the role of Alteplase?

It is a thrombolytic drug induce our body to produce an unwanted blood clotting dissolving substance.

Alteplase is used to treat a blood clot driven stroke and prevent death from acute myocardial infarction. It is also used to treat a blood clot in pulmonary embolism.

The mode of action of alteplase is that it dissolve blood clots which have formed in or around a catheter placed inside a blood vessel, leads to smoothen the flow of medicines injected in through the catheter.

Hence, alteplase use to bust the blood clot, provides perfusion, prevents further infarction, is the best statement.

Hence, Option A is correct.

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Your question was incomplete. The probable question was

A client is admitted to the emergency department with a diagnosis of myocardial infarction (MI). The primary health care provider (PHCP) prescribes the administration of alteplase. The registered nurse (RN) preceptor is orienting a new RN in the use of this medication. Which statement by the new RN indicates that teaching has been effective?

A. Administer the medication within 4 to 6 hours after onset of chest pain.

B. Assess for allergic reaction.

C. Check for signs of bleeding.

D. Give double the dose.

What is the difference between muscular endurance and muscular strength?

Answers

Answer:

Muscular strength is determined by how much force you can exert or how much weight you can lift. Muscular endurance refers to the ability of a muscle to sustain repeated contractions against resistance for an extended period of time

where and in which condition gold is found?​

Answers

Answer:

Gold is primarily found as the pure, native metal. Sylvanite and calaverite are gold-bearing minerals. Gold is usually found embedded in quartz veins, or placer stream gravel.

Answer:

gold minerals form in hot rocks in and around volcanoes.low sulfur,gold bearing hydrthermal fluids form when hot rocks heat ground water.gold is found in rivers and streams because water is the most efficient/fastest way of moving rocks.

I hope this helps

A 2-year-old unvaccinated boy is rushed to the emergency department with concerns for respiratory distress. His mother reports that he awoke in his usual state of health and was playing alone in the playroom when mom heard him have a coughing fit. Since that time, he has been choking and coughing, has had inspiratory stridor, and developed significant increased work of breathing. What is the most likely etiology of his stridor

Answers

Answer:

airway  obstruction from foreign body

Explanation:

The 2-year old was playing alone in the playroom. Children at this age are curious and it's likely he put something in his mouth and it's lodged in his throat, obstructing his aiway. The obstruction would explain the stridor, choking, and coughing. Telling you the child is unvaccinated is a distractor, it is important to recognize the child woke up healthy in his normal state of health.

Occupational Safety and Health Administration (OSHA) requirements apply to: (Select all that apply) A. Any employee with occupational exposure to pathogens. B. Hospitals only. C. Home health agencies. D. Medical and dental offices in which 10 or more people work.

Answers

Answer:

A

Explanation:

Psyllium is prescribed for the client diagnosed with a cardiac disorder to facilitate defecation and prevent straining with bowel movements. The nurse reinforces instructions to the client regarding administration of the medication. Which statement by the client indicates an understanding of the use of the medication

Answers

The statement by the client that indicates an understanding of the use of the medication will be "I SHOULD MIX THE MEDICATION WITH A FULL GLASS OF WATER."

PSYLLIUM is a type of medication that is mostly use in treating  a person or an individual that has constipation by helping to  increase  the amount of water in the stool, which is why it is often prescribed for a patient that had been diagnosed with a cardiac disorder which is a heart related disease by telling the patient TO  MIX THE MEDICATION WITH A FULL GLASS OF WATER in order to avoid or to prevent the patient from been choke from this medication which may lead to inability of the patient to breath, and to enables the disch:arge of faeces from the body  to be more easier  to pass.

Therefore The statement by the client that indicates an understanding of the use of the medication will be "I SHOULD MIX THE MEDICATION WITH A FULL GLASS OF WATER.

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It's recommended that those with osteoporosis __________. A. monitor their blood sugar levels throughout cardiorespiratory activities B. increase their bone density with high-intensity activities C. always have their inhalers close by during flexibility activities D. limit their overall activity time to 20 minutes per day Please select the best answer from the choices provided. A B C D

Answers

I believe that the answer is B, since osteoporosis affects the bone structure. Blood sugar levels should be monitored by people with diabetes while the inhaler is for asthma.

Answer:  Osteoporosis is a disease characterized by low bone mass and deterioration of bone tissue. Because of this bones become brittle and fragile.

Weight-bearing exercise is very important for maintaining bone health.

Therefore the correct answer is (B) increase their bone density with high-intensity activities .

Explanation:

A 4-year-old boy presents to your office with watery nonbloody diarrhea for the last three months. He has occasional abdominal pain prior to the onset of the diarrhea and his mother noted worsening flatulence, especially after meal times. Mom denies any recent travel or camping and the patient does not go to school or daycare. On exam, he is 60th percentile for weight and height (similar to his last well-child check), appears well, and has a normal abdominal exam. What is the most likely diagnosis

Answers

Lactase deficiency because Without enough of the lactase enzyme, your body can't metabolize dairy, leading to digestive problems like diarrhea, abdominal cramping or pain, bloating, gas, nausea, and sometimes even vomiting about 30 minutes to two hours after eating it.

Brainlist Question.

What is the role of doctors in the society. Explain.​

Answers

Answer:

I think the answer is Doctors are responsible for increased life expectancy and improved well-being in society. People who survive diseases such as cancer usually owe their survival to doctors, whose skills and dedication are vital for their cure.

Hope this helps you ^^

What is the bad cholesterol that is linked to high triglyceride levels, and what disease can it cause?

Answers

Answer:

High triglyceride levels also increase your risk of heart and vascular disease, including: Carotid artery disease. Coronary artery disease and heart attack. Metabolic syndrome (a combination of high blood pressure, diabetes and obesity).

Explanation:

Answer:

High LDL may cause CVA, MI

Explanation:

A client has presented to a clinic for the treatment of severe dermatitis after contact with poison ivy on a camping trip. The client has been prescribed prednisone, a corticosteroid, for the treatment of his skin condition. The client's care provider has emphasized that dosages of the drug will be gradually tapered off rather than stopped upon resolution of the symptoms. Which rationale is the most accurate for this dosing protocol

Answers

Answer:

Prednisone, a corticosteroid must be tapered slowly to avoid withdrawal symptoms of sudden steroid shortage.

Explanation:

while taking prednisione your adrenal glands will make less cortisol- which your body needs to function. if you stop the prednisone too quickly you will have withdrawal from the sudden stop of the medication.

apa itu biodiversiti

Answers

Answer: di mana kehidupan tertentu hidup misalnya Ikan yang tinggal di lautan Jadi pada dasarnya ia membuat beberapa kehidupan yang berbeza membentuk orang lain

Translation for anyone who wants to know: its where certain life lives for example A fish living in a ocean So basically it makes some life different form others

Answer:

halo semua saya mmm..

Explanation:

biosiversiti bagian dari biologi

What do you know about the disease epilepsy? Which is the treatment? ​

Answers

Epilepsy is a central nervous system disorder in which brain activity becomes abnormal, causing seizures or periods of unusual behavior, sensations, and sometimes loss of awareness.

Treatment: medications, sometimes surgery devices, or dietary changes can control seizures for the majority of people with epilepsy. Some people require lifelong treatment to control seizures, but for

It is possible to enhance the effects of regular physical activity by altering the duration, frequency, intensity, or progression of exercise. Match the situations to determine whether they are describing increasing duration, frequency, intensity, or progression of exercise: - Bill was swimming for 30 minutes, 3 days per week and recently increased that to 45 minutes, 3 days per week - Sonya was participating in one aerobics class per week and recently increased that to two aerobics classes per week - Erin was walking on the treadmill at a rate of 3 miles per hour for 60 minutes and recently was able to increase her rate to 4 miles per hour for 60 minutes - Chris used to do 30 minutes of cardio at 50% of his max heart rate 2 days per week and has gradually increased his performance such that now he is exercising for 60 minutes at 80% of his max heart rate 4 days per week A. Progression B. Intensity C. Frequency D. Duration

Answers

Answer:

 - Bill was swimming for 30 minutes, 3 days per week and recently increased that to 45 minutes, 3 days per week: D. Duration

- Sonya was participating in one aerobics class per week and recently increased that to two aerobics classes per week: C. Frequency

- Erin was walking on the treadmill at a rate of 3 miles per hour for 60 minutes and recently was able to increase her rate to 4 miles per hour for 60 minutes:  B. Intensity

- Chris used to do 30 minutes of cardio at 50% of his max heart rate 2 days per week and has gradually increased his performance such that now he is exercising for 60 minutes at 80% of his max heart rate 4 days per week: A. Progression

Explanation:

Duration refers to the amount of time accumulated in a specific physical exercise program (in this case, swimming), which is measured in a given time unit like minutes or hours. Moreover, the frequency can be defined as the occurrence/practice of a given physical activity (in this case, aerobics classes) that is repeated at specific intervals of time. Intensity refers to the speed/workload of a given exercise or training session. Intensity can be divided into two forms: 1-absolute intensity, which is expressed as a percentage of an individual's maximal aerobic capacity, and 2-relative intensity, which is the percent of one repetition maximum for each exercise. Finally, progression is the rate of increase of the intensity, frequency and duration of physical activity from the initial to the maintenance stages (the progression stages include initial, improvement and maintenance stages). Progression is dependent on diverse factors inherent to the individual, i.e., the tolerance to exertion, the objective of the physical exercise, and his/her age.

James is in his mid-40s and has little time to exercise as he runs a business. However, he knows that he should focus more on his health, eat healthy meals at regular intervals, and exercise as often as he can. One of the age-related conditions that James is most likely to face at his age is

Answers

Answer: Hypertension/High blood pressure

Explanation:

 James will likely develop Hypertension/High blood pressure. This is one the age-related conditions which is a cause of sudden frequent deaths.Now lack of exercise together with a bad diet can lead to obesity which  gives way to having high blood pressure and when not taken care of is a precursor to other diseases such as to heart disease, stroke, kidney failure and eventually death.  

Evidence from  research have proven that a Sedentary lifestyle or lack of physical activity together with a poor diet is a primary cause of chronic diseases.Apart from Hypertension/High blood pressure, there are also about  35 chronic conditions that can arise as people grow older  and some of them are type 2 diabetes, coronary heart disease, stroke, congestive heart failure, , deep vein thrombosis, cognitive dysfunction, depression and anxiety, osteoporosis, balance,  rheumatoid arthritis,  cancer, gallbladder disease,etc.

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b) How do ophthalmologists help sick people? ​

Answers

Answer:

by medicine

Explanation:

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Answer:

Ophthalmologist can diagnose and treat eye diseases through non-surgical means, such as by prescribing relevant medications

There are numerous recommendations from a variety of bodies regarding sodium intake. Match the mg of sodium with its corresponding recommendation: - The Adequate Intake (AI) value for sodium for men and women ages 9-50 years: - The Tolerable Upper Intake Level (UL) for sodium per day: - The amount of sodium the Percent Daily Value (%DV) is based on: - The amount of sodium needed per day to maintain physiological function: A. 200 mg B. 2,400 mg C. 1,500 mg D. 2,300 mg

Answers

Answer:

- The Adequate Intake (AI) value for sodium for men and women ages 9-50 years: C. 1,500 mg

- The Tolerable Upper Intake Level (UL) for sodium per day: D. 2,300 mg

- The amount of sodium the Percent Daily Value (%DV) is based on: B. 2,400 mg  

- The amount of sodium needed per day to maintain physiological function: A. 200 mg  

Explanation:

Sodium is a key dietary micronutrient that must be consumed daily in foods and beverages because the body is not able to generate sufficient quantities on its own. The World Health Organization (WHO) recommends a daily intake of 2 grams (2,000 mg) of sodium, whereas the American Heart Association (AHA) recommends 1,500 mg per day. Sodium is critical for regulating fluid levels in the body, muscle contraction, transmission of nerve impulses, and blood pressure. However, an excessive sodium intake can be harmful, because it holds extra fluid in the body, thereby creating an added burden on the heart and increasing blood pressure. In consequence, an excess of sodium in the diet may cause health problems such as heart diseases and stroke.

The USDA dietary guidelines recommend that individuals obtain approximately 0.80 grams of protein for each kilogram of body weight. This number should also fall between 10 and 35 percent of total daily calories. Jose weighs 152 pounds, which is a healthy weight for his height. He consumes about 2,200 calories a day. According to these USDA guidelines for daily protein intake, approximately how many grams of protein should Jose consume every day

Answers

Answer:

the correct answer is - 55 gm.

Explanation:

term definition of venous distension

Answers

A ramped rather in a white or white white or black or orange wood

A 26-year-old G2P1 woman at 41 weeks gestation is brought in by ambulance. The emergency medical technician reports that a pelvic examination performed 20 minutes ago when the patient had a severe urge to push revealed that she was fully dilated and the fetal station was 2. Fetal heart tones were confirmed to be in the 150s, with no audible decelerations. When the patient is placed on the fetal monitor, the heart rate is noted to be in the 60s. The maternal heart rate is recorded as 100. Without pushing, the fetal scalp is visible at the introitus. A repeat pelvic exam shows that the infant is in the occiput anterior position. What is the most appropriate next step in the management of this patient

Answers

Answer:

Have the mother push with her next contraction

Explanation:

the baby is in danger now with heart rate of 40. Mother is fully dilated and the baby is in the best (normal) position for labor. The baby needs to be delivered promptly.

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What is the equation of the graph outline the various challenges that you are likely to face during the implementation of a dam. Ai l Tng b th u tin ca ng? Evaluate the expression when x = 12/7The value of the expression when x equals is ???PLEASE HELP!! Find the value of each variable. When a solid dissolves in a liquid, it is termed Conference committees Group of answer choices must always include members from both political parties. must always include members from all three branches of government. are only likely to include members from both political parties when control of Congress is divided. are only likely to include members from all three branches of government when control of Congress is divided. Solve for 2. Round to the nearest tenth, if necessary.L58MAnswer: 2=Submit Answerattempt 1 out of 2PLS HELP Write choose the closest synonym for the word BELITTLE.(A) cleanse(B) criticize(correct answer?)(C) describe(D) shrink You witness a bank robbery, and follow the perpetrator down an alleyway. He stops at an orphanage and gives them all the money.Would you: A) Report the man to police since he committed a crime B) Leave him alone because you saw him do a good deed 12. Which of the following countries received economic aid from the Marshall Plan? The natural rate of unemployment is best defined as A. the state of the economy when there is zero unemployment. B. the rate of unemployment when there is no frictional or cyclical unemployment. C. the rate of unemployment when there is no cyclical or structural unemployment. D. the rate of unemployment after all workers and employers have fully adjusted to all changes in the economy. Question 3 of 10Which image shows an example of the strong nuclear force inaction?A.B.C.D. 2.According to Israelite belief, what was God's covenant with Abraham? What aspect of Chinese history has been a long standing feature? a. wealth b. emigration c. industrialization d. infrequent Anglo-Chinese wars Which equation describe the line through the points (2,-4) and (5,8) I NEED ANSWER ILL GIVE EXTRA POINTS AND EVERYTHING Which aspect of Mary Shelley's Frankenstein most clearly shows a Gothic influence in the story?A.Justine is wrongfully accused and convicted for the death of another character, William.B.The monster demands a life of peaceful existence in South America with a companion.C.The novel begins with a series of letters written by a British explorer of the Arctic.D.Frankenstein suffers mental torture from his guilt while his monster tortures others for vengeance. how would i find the area of the blue region and the perimeter of the outer window frame Which term of the AP. 8 , -4 , -16 , -28 ,......... is -880 ? hello there could use some help pls