A DNA fragment is introduced into the lacZ gene of a plasmid, which also contains an ampicillin resistance gene. What is the appearance of bacteria transformed with this plasmid if they are spread on plates containing ampicillin and X-gal

Answers

Answer 1

Answer: White colonies that are resistant to ampicillin.

Explanation:

The lac operon is an operon required for the metabolism of lactose in enteric bacteria such as Escherichia coli . It has three structural genes, a promoter, an operator and a regulator, all regulated by the availability of glucose and lactose. The lac repressor, a protein, senses lactose and blocks transcription of this operon. It acts as a repressor when lactose is present. A catabolite-activating protein (CAP), on the other hand, acts as a glucose sensor. The bacterium should express the lac operon only when lactose is available and glucose is not available. Thus, genes can always be transcribed, except when the Lac repressor protein is bound to the operon region, for which it has a high affinity (i.e. in the absence of lactose), where the Lac repressor protein maintains its high affinity for the operon region, preventing RNA polymerase from transcribing the structural genes. Thus, the system remains closed with consequent energy savings for the bacterium. In the presence of lactose, it binds to the Lac repressor protein and generates a conformational change that decreases its affinity for the operator region. Thus, the operator region is left free, RNA polymerase can freely transcribe the structural genes and the synthesized β-galactosidase (an enzyme) can degrade lactose to glucose plus galactose for energy.  

Thus, the lac z gene encodes the enzyme β-galactosidase, which catalyzes the hydrolysis reaction of lactose to glucose and galactose. In gene cloning experiments, a compound called X-gal is used as an indicator of cells expressing the β-galactosidase enzyme. X-gal is hydrolyzed by the enzyme to galactose and another compound that is oxidized giving an insoluble blue compound. Thus, if X-gal and a β-galactosidase inducer are dissolved in the medium of a culture plate where the transformed bacteria is found, colonies grown on the plate that possess a functional lac z gene (either because they were not transformed by the plasmid, or if they were but the plasmid does not have the cloned fragment or gene that disrupts the lac z gene) can be clearly distinguished by their blue coloration. If they have another gene inserted interrupting the lac z gene, they will not be able to produce the enzyme that degrades X-gal, resulting in white colonies since X-gal is not degraded giving that characteristic blue color.

The white, non-transforming colonies are eliminated by adding an antibiotic to the medium for which the plasmid provides resistance (in this case ampicillin), so that we can select the recombinant colonies that carry the vector with our sequence, simply by their color.

So, if bacteria are transformed with a plasmid (with ampicillin resistance) cloned with a gene that interrupts the lac z gene, the bacteria will be white because they do not synthesize the enzyme that degrades X-gal and will be resistant to ampicillin.


Related Questions

HELPPPPPPPPPPPPPPPP
HELPPPPP

Answers

Answer: 99% sure its 50

Explanation:

Answer:

Explanation:

I think 100

a funcao da membrana celular

Answers

Answer: Regulates the transport of materials entering and exiting the cell.

3. Shows like Sesame Street are part of the public health model of service delivery.

O True

O False


4. Which model of service delivery would focus most on getting a disabled person back into the workforce by focusing on what skills they still have or can develop?

O Medical model

O Public health model

O Social service model

O Human service model

Answers

Answer:

4. True

5. Social service model

A soil sample is 25 percent sand, 55 percent clay, and 20 percent silt. Use the following soil texture triangle to determine the type of soil present in this sample.

Clay
Silty loam
Sandy clay
Sandy clay loam

Answers

Answer:

Clay

Explanation:

Answer:

a: sample containing 30 percent sand, 35 percent clay, and 35 percent silt

Explanation:

which two organisms are closely related?

Answers

While orangutans and gorillas are in the great ape family, humans are most closely related to two other species in the family: bonobos and chimpanzees.

Carry learning!

study hard!

Brainliest please thank you!

Answer:

orangatangs and gorrilas are a prime example of close inheritance

Explanation:

These two species are the two closest related species

what are the differences between prokaryotic and eukaryotic nucleus

Answers

Answer:

Prokariotics neucleos is free floating while Eukariotics stay in place

Explanation:

Spelling is hard

How do i write a good hypothesis

Answers

Answer:

use "if...then...because"

Explanation:

a good way to write a hypothesis is using "if, then, because" statements because it fully explains your idea. you also need to make sure that your hypothesis is testable and realistic.

example/

Jimothy is testing if jazz music effects bee honey production. he writes this statement:

If jazz is played near a bee hive while producing honey, then the bees will produce more honey because the smooth music will raise their dopamine levels so that they become happier and therefore faster and more productive.

Answer:

I will write some tips in my explanation!

Explanation:

After formulating wh questions, it's time to form a hypothesis or an educated guess, but how do we do this?

First, you must gather the knowledge you already know; ad back it up with something that has happened like a study, article, etc

Example: It is a known fact that distilled water does not contain impurities, in fact, many distilled water companies boast about their water being clean and safe.

This will be the base of your hypothesis, and from here, just add the knowledge of which you know that can make up a well supported hypothesis, which you will then test.

Example:

It is a known fact that distilled water does not contain impurities, in fact, many distilled water companies boast about their water being clean and safe. Because distilled water is free of impurities, we can say that it cannot conduct electricity, but if we add salt to distilled water, the water will then be able to conduct electricity because...

That's my tips! If you are satisfied, please give a thanks or rate it! :D have a nice day

D D 4) After making an observation, what does a scientist ask?
What if I don't clean my room?
Are all doctors scientists?
A problem question.
What did someone else say about the same topic?
Who might disagree with me?

Answers

What did someone else say about the same topic

16. Use the graph to answer the questions.
a. What is the slope of the line?
b. What does it mean in the context of this graph?

Answers

Answer:

a) 1/2

b) It is gradually rising

Explanation:

a) To find the slope you do rise/run from the points given and in this case you go up one and over 2, therefore 1/2

the two pathways fundamentally involved in the physiological response to stress are

A. HPA and CRH
B. SAM and PVN
C. SAM and HPA
D. CRH and ACTH

Answers

Answer:

SAM and HPA

Explanation:

The physiology of stress has two components. One in which is a fast response meditated by the SAM axis, and one which is a slow response meditated by the HPA axis.

Print out the genetic code in the list of materials to answer this question.

Which amino acids are in the code AUGGUCGGU?


A)methionine, valine, and aspartic acid


B)methionine, valine, and glycine


C)arginine, histidine, and cysteine


D)methionine, glutamine, and alanine

Answers

Answer: methionine, vAline and glycine

Explanation:

3. How do animals survive in water and land habitat?

PLEASE HELP ME!!!!!!!​

Answers

Explanation:

A salamander adapted to its surroundings both on land & water. these creatures evolved to do both by living in in low- moisture & raining conditions.

Which of these is a helpful interaction that harms no animal?
Parasitism
Competition
Mutualism
Predation​

Answers

Answer:

Mutualism :)

Explanation:

Answer:

the answer is c

Explanation:

Mutualism means an ecological interaction between two living things.  Where they each get a benefit from the act. No one is harmed in the process.

Guys should I cut my hair

Answers

Explanation:

why ?????

Answer:

for me I will say no because you will not be as fine as now and why do you want to cut your hair

What is the haploid number for the Tasmanian devil?

Answers

Tasmanian devils have 14 haploid chromosomes

Why do sex-linked traits follow different patterns of inheritance than other traits ?

A. Females only have one X chromosome .
B. Males only have one X chromosome .
C. Males only carry recessive alleles .
D. Females only carry dominant alleles .

Answers

Answer:

Explanation:

X-linked genes have different inheritance patterns than genes on non-sex chromosomes (autosomes). That's because these genes are present in different copy numbers in males and females.

PLEASE HELP WITH THIS ONE QUESTION
Which statement about oogenesis is correct?

A) Diploid secondary oocytes undergo meiosis II to form a haploid ootid cell and a polar body.

B) Haploid secondary oocytes undergo meiosis II to form a diploid ootid cell.

C) Two diploid secondary oocytes undergo meiosis II to form four haploid ootid cells.

D) Haploid secondary oocytes undergo meiosis I to form a haploid ootid cell and a polar body.

Answers

Answer:

A is correct.

Explanation: At the end of meiosis II there are 3 polar bodies and one octet that are all haploid.

Write a one-paragraph (5-7 sentences) reflection on the dangers of drugs and how they can interfere with you achieving your goals.

Answers

Answer:

They can reflect on getting a Job and doing good in life, they could kill a person without them even knowing it. The dangers of drugs Is that they can mess up your life or it could make you so messed up things to yourself. Drugs are bad because they could make life worse, and they could hurt people around you or people you love, drugs can lower your chances of achieving your goals because you could easily get fired or you can lose your mind enough just not to know what you're doing.

Explanation:

Question 1: One of the central themes in biology is how DNA, RNA, and proteins are related. Describe how genetic information flows between those types of molecules. Include the results of the processes of transcription and translation? ( Will Mark Brainliest if answered correctly. Do Not Repost Someone Else's Answer that is already on Brainly or any other websites please). ​

Answers

Answer:

Translation is the process by which mRNA is decoded and translated to produce a polypeptide sequence, otherwise known as a protein. This method of synthesizing proteins is directed by the mRNA and accomplished with the help of a ribosome, a large complex of ribosomal RNAs (rRNAs) and proteins. In translation, a cell decodes the mRNA’s genetic message and assembles the brand-new polypeptide chain. Transfer RNA, or tRNA, translates the sequence of codons on the mRNA strand. The main function of tRNA is to transfer a free amino acid from the cytoplasm to a ribosome, where it is attached to the growing polypeptide chain. tRNAs continue to add amino acids to the growing end of the polypeptide chain until they reach a stop codon on the mRNA. The ribosome then releases the completed protein into the cell.

Answer:

DNA is the code that stores all our genetic information. When making a protein, DNA has to go through transcription and then translation. In transcription, an the genetic code of DNA is copied to mRNA, with the aid of an RNA polymerase protein which attaches single RNA units to the mRNA strand as the RNA polymerase moves on the DNA. Note that instead the letter T, RNA contains U. This occurs in the nucleus. The mRNA travels to the cytoplasm to a ribosome. The RNA also trims itself, removing parts that it does not need, and keeping parts for translation. The ribsomes use tRNA attached to amino acids to assemble the protein chain and translate the mRNA into proteins. As the amino acid chain, or polypeptide forms, a protein comes to be. However, there may be mutations, resulting from translation or transcription and sometimes these mutations may cause harmful affects.

help :(
Which of the following is the most accurate example of supply and demand?


The consumer cost for roses drops when fewer roses are available for sale.

The price of rice increases as more of it is produced.

The consumer cost for roses drops when fewer roses are available for sale.

The large amount of unemployed farm laborers drops farm wages.

Answers

Answer:

Which of the following is the most accurate example of supply and demand?

The consumer cost for roses drops when fewer roses are available for sale.

Explanation:

What is demand?

Demand refers to consumers' desire to purchase goods and services at given prices. Demand can mean either market demand for a specific good or aggregate demand for the total of all goods in an economy.

The consumer cost for roses drops when fewer roses are available for sale.

Explanation:-

Demand means the asking of consumers for the product.If demand of any product increases among consumers the price also increases.If demand of any product decreases among consumers the price also decreases .

[tex]\\ \sf\longmapsto Demand\propto Product [/tex]

How are root hair cells adapted for it's function?

Answers

The microscopic root hairs in plants are well adapted for their functions.

The root hairs are contained in plants. These root hairs are very small growths at the root of plants that can only be seen with the aid of a powerful microscope.

The following are the adaptations of root hairs;

They have a large surface area.The have a lot of mitochondria to enable active transport.

Hence, the microscopic root hairs in plants are well adapted for their functions.

Learn more about root hairs: https://brainly.com/question/16782990

3. Label the diagram with the following words
DNA
Cell
Gene
Chromosome
nucleus
Base Pair

!!!!!!
(Don’t worry about number 2)

Answers

Answer:

ok so i think the X shape is a chromosome, that blank space in the big spot is calleed a nucleas. The arrow on the top right is a cell, the bottom right is base pair, the bottom left is dna and the top middle is called the gene.

Explanation:

Applying the systems thinking techniques, outline the practical steps that can be taken to address drought

Answers

Some practical steps to address drought may include the implementation of efficient water usage techniques, the protection of vegetation, and the use of methods to retain moisture in the soil.

What is a drought?A drought is classified as the shortage or absence of water for a prolonged period of time in a region. These can be caused by a prolonged lack of rainfall.Other factors include poor water management and lack of vegetation.

Methods to avoid droughts.The planting and protection of vegetation, primarily those adapted to dry environments, is important in preventing drought.Furthermore, the correct implementation of efficient water usage techniques can aid in preventing drought by limiting the amount of water that is wasted. Lastly, the use of mulch and other water retainers can be an effective method in keeping the soil moist and aids in the previous two methods.

Therefore, we can confirm that the most effective ways to avoid droughts are to practice efficient water use so as to not waste water, to plant and protect vegetation, and to use mulch and other moisture-retaining methods to keep the soil from drying.

To learn more about droughts visit:

https://brainly.com/question/2791517?referrer=searchResults

Why can't we just use centuries, decades

Answers

We can? Weeee in them rn

What characteristics in a human pedigree suggest a mitochondrial location for a mutation affecting the trait

Answers

Answer:

maternal inheritance

Explanation:

the differing levels of expression of the mutant phenotype in different progeny

How many genes are shared by all viruses?

Answers

Answer:

200 approximate

Explanation:

depends how many in common

What is mars' origin of the name?

Answers

Answer:

Mars was named by the ancient Romans for their god of war because its reddish color was reminiscent of blood

Explanation:

The origin of the name of Mars was named by the ancient Romans for their god of war because its reddish color was reminiscent of blood.

During the Green Revolution pesticides
were
A. overused
B. all organic
C. used sparingly
D. all natural

Answers

From researching, the answer might be A. Overused

Answer: A. overused

Explanation:

Organisms that live where there is no
light use a process that converts energy
from chemicals into sugars. What is it
called?
A. photosynthesis
B. dark reactions
C. chemosynthesis
D. chemical bonding

Answers

Answer:

c

Explanation:

Answer:

c

Explanation:

Chemosynthesis is the synthesis of organic compounds by bacteria or other living organisms using energy derived from reactions involving inorganic chemicals, typically in the absence of sunlight.

Mutations are not always negative. Explain why this statement is true. Provide two reasons using examples to support your answer.

Answers

2 examples -
1.) in italy, ( limone ) a unique mutation protects them from developing atherosclerosis,
2.)theres mutation in bacteria too, this mutation allows bacteria to survive in the presence of antibiotic drugs, leading to the evolution of antibiotic-resistant strains of bacteria.
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